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目的:观察艾灸对类风湿关节炎(RA)模型大鼠滑膜组织中Ras、细胞外信号调节激酶1/2(extracellular signal-regulated kinase1/2,ERK1/2)和Raf蛋白表达的影响,探讨艾灸治疗类风湿性关节炎的分子作用机制。方法:选取30只Wistar大鼠,随机分为正常组、模型组、艾灸组,每组10只。采用风、寒、湿环境因素+生物因子(弗氏完全佐剂)复合造模。造模后第3天进行治疗,艾灸组取双侧 "肾俞""足三里"穴悬灸,20 min/次,1次/d,连续治疗15 d。于造模前、造模后及治疗第15天分别测量各组大鼠右后足趾容积。Western blot法检测滑膜组织中Ras蛋白、ERK1/2、磷酸化ERK1/2(phosphor-ERK1/2,p-ERK1/2)及Raf蛋白的相对表达量。结果:造模后大鼠右后足趾容积较正常组明显增大(P<0.01);经过15 d治疗,艾灸组大鼠的跖围小于模型组(P<0.01)。模型组Ras、Raf、ERK1/2、p-ERK1/2蛋白表达较其他各组增加(均P<0.01);艾灸组Ras、Raf、ERK1/2、p-ERK1/2蛋白表达明显低于模型组(均P<0.01)。结论:艾灸能降低类风湿性关节炎模型大鼠滑膜组织中Ras、Raf及ERK1/2的过度表达,进而抑制Ras-MAPK信号通路的异常活化,推断这可能是艾灸减轻滑膜炎性反应的分子机制之一。
Abstract：Objecive To observe influence of moxibusion at "Shènshù" (肾俞 BL 23) and "Zúsānlǐ " (足三里 ST 36) on proteins expression of Ras, ERK1/2, and Raf in synovial issues in rats with rheumatoid arthriis (RA) to elucidate molecular mechanisms of moxibusion on treaing RA. Methods Thirty Wistar rats were randomly divided into a normal control group (Group Cont), a model group (Group Mod) and a moxibustion group (Group Mox) with 10 rats in each group. The model was established by using both environmental factors such as wind, cold and damp and a biological factor (Freund’s complete adjuvant). Suspended moxibusion was applied at bilateral BL 23 and ST 36 for 20 min, applied once daily for 15 days, starting from the 3rd day after modeling. Volumes of metaleg digii pedis on the right side were measured before, ater and 15 days after modeling. Protein expressions of Ras, ERK 1/2, p-ERK1/2 and Raf in synovium tissues were detected by western blot. Results After modeling, volumes of metaleg digii pedis on the right side were significantly enlarged, compared with those of rats in Group Cont (P<0.01). Ater treatment by moxibustion for 15 days, the metatarsale girth of metaleg digiti pedis on the right side in Group Mox was significantly reduced, compared with that in Group Mod (P<0.01). Protein expressions of Ras, Raf, ERK1/2 and p-ERK1/2 in Group Mod were significantly increased, compared with those in Group Cont (P<0.01) and Group Mox (P<0.01). Protein expressions of Ras, Raf, ERK1/2 and p-ERK1/2 in Group Mox were significantly lowered, compared with those in Group Mod (all P<0.01). Conclusions Moxibusion can suppress excessive protein expressions of Ras, Raf and ERK1/2 in synovium tissues of rats with experimental RA to inhibit abnormal acivaion of Ras-MAPK signal Pathway, which may be one of the molecular mechanisms of moxibusion in alleviaing synoviis.
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基本信息:
中图分类号:R245
引用信息:
[1]姚剑,胡玲,宋小鸽,等.艾灸“肾俞”“足三里”穴对类风湿关节炎大鼠滑膜组织中Ras-MAPK信号通路的影响(英文)[J].World Journal of Acupuncture-Moxibustion,2013,23(02):29-33.
基金信息:
Supported by Naional Key Basic Research Program(973, No. 2009 CB 522905)
2013-06-30
2013-06-30